Fig. 7.

In vivo efficacy of CD38pep- and CD138pep-targeted nanoparticles loaded with pro-drug doxorubicin. Nanoparticles targeted with CD38pep or CD138pep were prepared loaded with a doxorubicin pro-drug, and their in vivo efficacy was tested against that of free doxorubicin in a subcutaneous xenograft mouse model. Mice were injected with H929 cells and tumors allowed to grow to a predetermined size before IV injection of nanoparticle formulations began on day 1. Mice were injected with 3 mg/kg of doxorubicin or nanoparticle pro-drug equivalent on days 1, 3, 5, 7, and 9. Tumor volume (a), survival (b), and mouse weight (c) were tracked with mice being killed when tumor volume grew too large or mouse weight too low. After death or at the end of the study, organs were dissected and weighed (d). n = 6 for all groups and data represents means (± s.e.)