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. 2019 Dec 29;48(4):0300060519883098. doi: 10.1177/0300060519883098

Figure 2.

Figure 2.

Upregulation of miR-147a significantly reduces NSCLC cell aggressiveness: (a) NSCLC cell lines H1650 or H1299 were incubated with different concentrations of miR-147a (10, 30, 50, 80, or 100 µmol/L) for 24 hours, and colony formation and wound healing assays were performed. The images shown are representative of three experiments, (b) H1299 and H1650 cells were transfected with miR-147a mimic or miR-147a inhibitor and the level of miR-147a was determined by qRT-PCR, (c) H1299 or H1650 cells were transfected with FAM-labeled miR-147a and transfection efficiency was measured after 24 hours by fluorescence microscope. The transfected H1650 and H1299 cells expressed green fluorescence, indicating high efficiency of miRNA transfection, (d) H1299 and H1650 cells were transfected with miR-147a mimic or miR-147a inhibitor and subjected to CCK-8 assay, (e) H1299 and H1650 cells were transfected with miR-147a mimic or miR-147a inhibitor, and migration of NSCLC cells was determined by wound-healing assay, (f) H1299 and H1650 cells were transfected with miR-147a mimic or miR-147a inhibitor, and invasion of NSCLC cells was determined by Transwell assay and (g) H1299 or H1650 cells were transfected with miR-147a mimic or treated with miR-147a inhibitor, and colony formation of H1650 and H1299 cells was detected. **P < 0.01, compared with control. NSCLC, non-small-cell lung cancer; qRT-PCR, quantitative real-time PCR; OD, optical density. Values are shown as mean ± SD.