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. 2020 Nov 3;7:52. doi: 10.1186/s40662-020-00217-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — TGFβ3 knockdown in human CSSC. a Human CSSC were transfected with hTGFβ3 siRNA or scrambled sequences. After co-culture with RAW cells under M1 phase in a Transwell system for 48 h, naïve hCSSC and hCSSC transfected with scrambled sequences showed similar TGFβ3 upregulation, whereas cells with TGFβ3 siRNA transfection showed reduced expression (decreased by 70–84%) of hTGFβ3. Similar results were obtained in two separate experiments. b The reduced hTGFβ3 expression was specific for si-TGFβ3 transfection, while there was no changes for scrambled sequences. The expression of hTGFβ1 remained unchanged with TGFβ3 knockdown in CSSC. Mean and SD are calculated from triplicate experiments