Table 1.
Fluorescent Dyes Used in Clinical Applications.
| Clinically approved dyes | |||||
|---|---|---|---|---|---|
| Compound | Excitation (nm) | Emission (nm) | Quantum yield (%) | Molar absorption coefficient (L*Mol-1*cm-1) | Brightness |
| Fluorescein2,3 | 488 (PBS) |
515 (PBS) |
12 (HSA) |
40000 (HSA) |
4800 |
| ICG4 | 807 (FBS/HEPES) | 822 (FBS/HEPES) |
9,3 (FBS/HEPES) |
121000 (FBS/HEPES) |
11253 |
| Methylene blue4 | 665 (FBS/HEPES) | 688 (FBS/HEPES) |
9,6 (FBS/HEPES) |
49500 (FBS/HEPES) |
4752 |
| PpIX5-ALA/HAL 5-7 | 404 (PBS) | 634 | 4,1 (HSA) |
4866 | 200 |
| Dyes applied in clinical studies | |||||
| Compound | Excitation (nm) | Emission (nm) | Quantum yield (%) | Molar absorption coefficient (L*Mol-1*cm-1) | Brightness |
| Cy58,9 | 650 (PBS) |
667 (PBS) |
27 (PBS) |
250000 (PBS) |
67500 |
| Cy79-11 | 750 (PBS) |
777 (PBS) |
2,6 (PBS) |
200000 (PBS) |
5200 |
| IRDye800CW12,13 | 774 | 788 | 14,2 (FBS/HEPES) |
237000 | 33654 |
| SO45614,13 | 776 | 793 | 15,1 | 272000 | 41072 |
| IRDye 700DX15,16 | 689 | 700 | 14 | 170000 | 23800 |
| SGM-101 (BM-104)17,18 |
685 (pH 6 buffer) |
705 (pH 6 buffer) |
17 (Buffer) |
N.R. | N.A. |
| ZW800-I19,13 | 772 | 788 | 15,1 (FBS/HEPES) | 249000 | 37599 |
NR=not reported, N.A. = not available, could not be calculated.