Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The growth defect of a trm8Δ pus7Δ mutant at high temperature is not complemented by expressing pus7-D478Y. Wild type and trm8Δ pus7Δ strains harboring [CEN URA3 PPUS7-pus7-D503Y] or vector as indicated were grown overnight in S-Ura medium containing dextrose, diluted to OD600 of approximately 0.2 and serially diluted 10-fold, and then 2 μL was spotted onto YPD media, followed by incubation for 3 days at temperatures as indicated.
