Fig. 7.

Double immunofluorescence photomicrographs of the medial preoptic (PONm) and lateral hypothalamic (LHN) nuclei of E. bruniceps showing the association between CART- (green) and TH (red)-containing systems. Low (A–C; G–I) and high (D–F; J–L) magnification photomicrographs showing CART- and TH-immunoreactive cells and fibers in PONm and LHN. None of the CART neurons in the PONm and LHN contain TH (C, F, I, L). v ventricle; VMN nucleus ventromedialis hypothalamic. Scale bar = 50 µm in A–C and G–I, 25 µm in D–F and J–L