Fig. 6.

Knockdown of Nrf2 expression attenuated the protective effects of GAS on H₂O₂-treated H9c2 cells. a RT-PCR results showing Nrf2 mRNA expression levels in negative control cells (cells treated with nontargeting siRNA) and cells treated with Nrf2-siRNA. b Western blotting results showing Nrf2 protein expression levels in negative control cells and Nrf2-siRNA-treated cells. c Viability of negative control cells or Nrf2-siRNA-treated cells subjected to 400 μM H₂O₂ treatment with or without GAS pretreatment. d Intracellular ROS levels of negative control cells or Nrf2-siRNA-treated cells subjected to 400 μM H₂O₂ treatment with or without GAS pretreatment. The data presented are the mean ± SD of three independent experiments. *P < 0.05 between the indicated two groups