Table 2.
Metabolism of alflutinib by recombinant human P450 isoforms
| Name | Retention time (min) | Elemental composition | Relative MS area (%) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1A2 | 2A6 | 2B6 | 2C8 | 2C9 | 2C19 | 2D6 | 2E1 | 3A4 | 3A5 | |||
| M0 | 9.36 | C28H31F3N8O2 | 93.69 | 95.15 | 95.84 | 95.02 | 91.04 | 96.18 | 96.27 | 95.02 | 21.07 | 66.58 |
| M224 | 5.74 | C13H12N4 | — | — | 1.50 | 0.74 | 3.36 | 0.73 | 2.46 | 1.93 | 38.81 | 10.14 |
| M484 | 10.3 | C23H19F3N6O3 | — | — | — | — | — | — | — | — | 2.38 | 0.61 |
| M486 | 6.9 | C26H30N8O2 | 0.47 | 1.36 | 0.74 | 0.47 | 1.03 | 1.19 | — | 0.65 | — | 0.38 |
| M497 | 9.94 | C24H22F3N7O2 | — | — | — | — | — | — | — | — | 0.66 | — |
| M554-1 | 7.78 | C27H29F3N8O2 | 0.35 | 0.68 | 0.48 | 0.35 | 0.60 | 0.28 | 0.22 | 0.52 | 0.81 | 0.57 |
| AST5902 | 9.09 | C27H29F3N8O2 | — | — | — | 1.35 | 1.79 | 0.19 | — | 0.22 | 9.80 | 5.93 |
| M555 | 8.94 | C26H24F3N7O4 | — | — | — | — | — | — | — | — | 0.74 | — |
| M584-1 | 8.14 | C28H31F3N8O3 | 4.66 | — | — | 1.19 | 0.37 | 0.72 | — | — | 0.50 | 3.75 |
| M584-2 | 8.59 | C28H31F3N8O3 | — | — | — | — | — | — | — | — | 1.26 | 2.44 |
| M584-3 | 9.19 | C28H31F3N8O3 | 0.66 | 2.81 | 1.45 | 0.55 | 1.81 | 0.72 | 0.78 | 1.45 | 3.56 | 2.08 |
| M586 | 6.92 | C27H29F3N8O4 | — | — | — | — | — | — | — | — | 6.25 | 0.98 |
| M600 | 7.11 | C28H31F3N8O4 | 0.17 | — | — | — | — | — | 0.27 | 0.22 | 14.14 | 6.55 |