Figure 1.

Development of systemic symptoms in maize seedlings infected with SCMV. A, Infection progress and mosaic/streaking symptoms on the first and second systemically infected leaves of SCMV-inoculated maize seedlings and equivalent leaves of mock-inoculated plants (Mock) at 3, 5, 7, and 9 dpi. The white dashed boxes indicated regions showing mosaic/streaking symptoms as they expanded between 5 and 9 dpi. Scale bars = 0.5 cm. B, Levels of photosynthetic pigments in the first systemically infected leaves over the course of infection. Data were presented as means ± se (n = 3 to 5). Asterisks indicate the significant differences between mock-inoculated and SCMV-infected plants as evaluated by two-tailed Student’s t tests. C, Accumulation of SCMV RNA and CP levels in the first systemically infected leaves of mock-inoculated and SCMV-infected maize seedlings at 3 and 9 dpi. RT-qPCR data (top) are presented as means ± se (n = 4) and relative to ZmUbi (ubiquitin). Asterisks indicate significant differences between 3 and 9 dpi in SCMV-infected plants determined by the two-tailed Student’s t test. Immunoblots (bottom) show SCMV CP (arrowhead) accumulation at 3 and 9 dpi. Actin was used as a loading control.