Figure 6.

Overexpression or knockdown of AtNFS1 and AtFH does not affect shoot dark respiration of 4-week-old Arabidopsis plants. Arabidopsis wild-type (WT; Col-0), atfh1, AtFH-16-OX, atnfs1-7, AtNFS1.2-18-OX, AtNFS1.1-1-OX, and atogdh2-2 seeds were germinated and seedlings were grown in solid one-half strength MS-agar media. The ogdh2-2 mutant was used as a positive control of respiration because it has been shown to reduce dark respiration. After 5 d of growth, evenly germinated seedlings were transferred to a Falcon 6-well culture plate containing liquid one-half strength MS media. Ten plants were pooled together per well to make a biological replicate with a total of five biological replicates per genotype. After another 18 d of growth in liquid media, the plants were transferred to darkness 1 h before CO₂ flux measurements using a LI-850 CO₂/water gas analyzer. Error bars represent se of five biological replicates from two different experiments. Asterisks denote statistical significance according to Student’s t test (**P < 0.01). Experiments were repeated two times with similar results.