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. 2020 Sep 9;184(3):1573–1584. doi: 10.1104/pp.20.00528

Figure 1.

Figure 1.

SBTs are involved in CLE40 propeptide processing. A, The activity of eCLE40 is reduced in plants expressing EPI1a and EPI10 SBT inhibitors compared with wild-type (wt) plants. Two independent transgenic pCLE40:EPI1a and pCLE40:EPI10 lines (1 and 2) and wild-type plants were grown for 5 d on plates containing 0 (control), 1 µm, or 10 µm eCLE40. Root length is shown in percentage of the no-peptide control (means ± se). Asterisks indicate significant differences between eCLE40-treated transgenic and wild-type plants using two-tailed unpaired Student’s t test (***P < 0.001; n ≥ 12). B, Expression of four SBT genes is regulated by eCLE40 treatment. SBT expression was analyzed in 5-mm root tips by quantitative real-time PCR (qPCR). Relative expression levels are shown as fold change in eCLE40-treated plants compared with untreated controls. Asterisks indicate significant differences from the control using Student’s t test (* P < 0.05, **P < 0.01, and ***P < 0.001; n ≥ 3).