Figure 6.

Impact of P4A and T5A substitutions on CLE40 structure and activity. A, Peptide structure predictions suggest that Ala substitutions for P4 and T5 may have an impact on peptide conformation. Peptides are shown from the N terminus (blue) to the C terminus (red); amino acids in positions 4 and 5 are indicated. B and C, Bioactivity (inhibition of root growth) is not affected by P4A and T5A substitutions. B, Representative 6-d-old Arabidopsis seedlings grown on medium containing 1 µm matCLE40 (mat), the P4A and T5A substituted peptides, or no peptide as a control. C, Root length of peptide-treated as compared with control seedlings (means ± se; n ≥ 50). Asterisks indicate statistically significant differences from the control using (***P < 0.001).