Skip to main content
. 2020 Sep 1;184(3):1263–1272. doi: 10.1104/pp.20.00997

Figure 4.

Figure 4.

RAM1 overexpressors show altered shoot development and constitutively express root AM marker genes in their shoots. A, Photograph of 4.5-week-old B. distachyon plants transformed with 35S:NLS-GFP or 35S:BdRAM1. The three independent transformant lines overexpressing BdRAM1 (ox1, ox2, and ox3) display a bushy stature, whereas the 35S:BdRAM1-transformant line not overexpressing BdRAM1 (wild type [WT]) resembles the 35S:NLS-GFP control plant. B, Gene expression of BdRAM1 and of several root AM marker genes in shoots. All tested genes are strongly induced in 4.5-week-old shoots of three 35S:BdRAM1ox lines (ox1, ox2, and ox3) relative to control plants transformed with 35S:NLS-GFP (GFP) or the 35S:BdRAM1-transformant line not overexpressing BdRAM1 (wild type). Gene expression was measured by RT-qPCR. Bar graphs show the mean, with error bars representing sd. Single points represent individual measurements. Significance values (ANOVA) for each gene are indicated in the figure. Pairwise comparisons were conducted using Tukey’s HSD post-hoc test. Different lowercase letters denote significant differences.