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. 2020 Sep 2;184(3):1585–1600. doi: 10.1104/pp.20.00604

Figure 7.

Figure 7.

Phosphorylation of HDA15 on Ser-448 and Ser-452 is critical for enzymatic activity and oligomerization. A, Size exclusion chromatography of the phosphorylation site mutants of HDA15ZFHD. B, The ribbon diagram model showing how phosphomimetics of HDA15 on S448 and S452 may regulate its enzymatic activity and oligomerization. C, Fluorometric HDAC activity assays of the phosphorylation site mutants of GFP-HDA15 in Arabidopsis. Proteins were extracted from 10-d-old etiolated hda15-1 seedlings expressing truncated HDA15. Immunoblotting with the GFP antibody indicated the protein loading in HDAC activity assays. Data represent the mean (± sd) of three biological replicates. Asterisks indicate significant difference using Student’s t test (**P < 0.01). D, Relative fluorescence of protochlorophyllide in full emission wavelengths 600 to 800 nm in etiolated transgenic plants expressing phosphorylation site mutants of GFP-HDA15. Protochlorophyllide was extracted from 4-d-old etiolated seedlings. E, RT-qPCR analysis of GUN5 and PSBQ expression in 2-d-old etiolated transgenic seedlings expressing phosphorylation site mutants of GFP-HDA15. UBQ10 was used as an internal control. Data represent the mean (± sd) of three biological replicates. Asterisks indicate significant difference using Student’s t test (*P < 0.05). WT, wild type.