Figure 8.
Subcellular localization and oligomerization of HDA15 phosphorylation status. A, Subcellular localization of GFP-HDA15 and mutants with S448 and S452 changed into Asp (D) or Ala (A) in Nicotiana benthamiana (scale bar = 15 μm) and Arabidopsis (scale bar = 10 μm). mCherry carrying a nuclear localization signal was used as the nuclear marker. Cell walls were stained by propidium iodide. B, bimolecular fluorescence complementation (BiFC) assays of Arabidopsis protoplasts showing the oligomerization of HDA15 and the mutants with S448 and S452 changed into Asp (S448S452D) or Ala (S448S452A). Wild-type (WT) and mutated HDA15 fused with the C terminus (YFPC) or the N terminus (YFPN) of YFP were cotransfected into protoplasts and visualized by confocal microscopy. Scale bar = 10 μm. DIC, Differential interference contrast.