Table 1. Arabidopsis class II HDACs oligomerization and enzyme kinetics.

Proteins	Oligomer State	[E]	Vmax	Km	kcat	kcat/Km
		nm	μm s−1	μm	S−1	μm−1 S−1
Wild type
HDA5HD	Monomer	25	0.65 ± 0.06	91.5 ± 26.0	26 ± 2.4	0.28
HDA18HD	Monomer	25	0.25 ± 0.01	47.7 ± 7.63	10 ± 0.4	0.2
HDA15HD	Monomer	75	2.31 ± 0.85	13.95 ± 4.15	30.82 ± 11.28	2.22
HDA15HD	Tetramer	25	1.02 ± 0.13	6.09 ± 1.49	40.73 ± 5.32	7.37
HDA15ZFHD	Dimer	2.5	3.9 ± 0.49	13.75 ± 0.89	1558.08 ± 97.71	113.78
Interface mutation
HDA15ZFHD E163A	Dimer	2.5	3.01 ± 0.2	20.02 ± 4.24	1205.83 ± 79.5	63.35
HDA15ZFHD E165A	Dimer	2.5	3.42 ± 0.45	14.5 ± 1.87	1367.99 ± 79.66	95.61
HDA15ZFHD Q169A	Dimer	2.5	3.09 ± 0.15	19.02 ± 0.99	1236.56 ± 58.84	65.25
HDA15ZFHD Y228A	Dimer	2.5	3.25 ± 0.14	18.59 ± 3.39	1299.43 ± 57.21	71.83
HDA15ZFHD Y230A	Dimer	2.5	2.41 ± 0.25	21.94 ± 3.25	964.74 ± 99.34	44.45
HDA15ZFHD Y228Y230A	Monomer	2.5	1.93 ± 0.03	35.65 ± 4.56	772.95 ± 11.58	22.02
HDA15ZFHD E173A	Monomer	25	1.49 ± 0.45	26.29 ± 6.83	59.42 ± 17.85	2.3
HDA15ZFHD R177A	Monomer	625	2.39 ± 0.15	75.32 ± 24.64	3.83 ± 0.24	0.06
Phosphorylation mutation
HDA15ZFHD S448D	Monomer	25	4.48 ± 0.14	13.35 ± 1.5	179.2 ± 5.6	13.42
	Dimer	2.5	2.58 ± 0.16	35.48 ± 5.04	1032 ± 64	29.08
HDA15ZFHD S452D	Monomer	25	5.26 ± 0.12	11.71 ± 1.0	210.4 ± 4.8	17.96
	Dimer	2.5	3.08 ± 0.08	11.65 ± 1.32	1232 ± 32	105.75
HDA15ZFHD S448S452D	Monomer	25	3.89 ± 0.17	27.39 ± 3.1	155.6 ± 6.8	5.68
	Dimer	25	3.5 ± 0.09	21.17 ± 1.69	140 ± 3.6	6.61
HDA15ZFHD S448A	Dimer	2.5	4.52 ± 0.17	18.12 ± 2.16	1808 ± 68	99.78
HDA15ZFHD S452A	Dimer	2.5	4.69 ± 0.12	12.13 ± 1.27	1876 ± 48	154.66
HDA15ZFHD S448S452A	Dimer	2.5	4.68 ± 0.2	17.58 ± 2.44	1872 ± 80	106.48