Figure 5.

Detection of WHY2 triple localization during plant aging. A, Indication of three stages of wild-type (WT) plant development [4, 6, and 8 weeks (W)] by visible rosette leaf senescence. B, Indication of three stages of wild-type plant development (4, 6, and 8 weeks) and photosystem II fluorescence efficiency (Fv/Fm) and chlorophyll content. Error bars represent the sd of six biological replicates. C, WHY2 protein distribution among mitochondrion, chloroplast (Chl), and nucleus by immunodetection. Anti-PSII was used as a chloroplast protein control, anti-H3 as a nuclear protein control, and anti-VDAC1 (voltage-dependent anion channel 1) as a mitochondrial protein control. Silver staining of the protein gel was used to indicate loading.