Figure 1.
XRN4 differentially accumulates in polysomes during seedling development. A, Polysomal extracts prepared from 3-, 7-, 15-, and 25-d-old seedlings were fractionated on a Suc gradient, and polysome traces were obtained through measurement of OD254nm. Polysome profiling was performed starting from identical quantities of N2-pulverized tissues (e.g. 300 mg of biomass). B, Total proteins extracted from polysomal and input fractions were analyzed by immunoblotting. The four blots were probed with an antibody specific to XRN4. Inputs correspond to an equivalent of 10 mg of tissue powder for all stages. For polysomal fractions, loaded proteins were precipitated from identical volumes of each fraction. Data are representative of at least three replicates.