Metagene analyses displaying the abundance of 5ʹP reads relative to stop codons. B, Wild-type (wt) 3-d-old stage. C, Wild-type 7-d-old stage. D, Wild-type 15-d-old stage. E, Wild-type 25-d-old stage. F, xrn4-5 3-d-old stage. G, xrn4-5 7-d-old stage. H, xrn4-5 15-d-old stage. I, xrn4-5 25-d-old stage. Data are means ± sd. The illustrations (A) represent 5ʹP intermediate accumulation at –47 and –17 nucleotides (nt) before stop codons. RPM, Reads per million.