Table 3.
Antibodies used in Western blotting and immunohistochemical staining
| Targets | Source | Species reaction | Usage |
|---|---|---|---|
| COL-1 | Abcam, ab6308 | Mouse-anti-human | WB |
| FN | Abcam, ab45688 | Rabbit-anti-human | WB |
| CTGF | Abcam, ab209780 | Rabbit-anti-human | WB |
| Erk | Abcam, ab32537 | Rabbit-anti-human | WB |
| p-Erk | Abcam, ab131438 | Rabbit-anti-human | WB |
| P38 | Abcam, ab170099 | Rabbit-anti-human | WB |
| p-P38 | Abcam, ab47363 | Rabbit-anti-human | WB |
| JNK | Abcam, ab110724 | Rabbit-anti-human | WB |
| p-JNK | Abcam, ab47337 | Rabbit-anti-human | WB |
| Smad 2/3 | Abcam, ab202445 | Rabbit-anti-human | WB |
| p-Smad2 | Abcam, ab63399 | Rabbit-anti-human | WB |
| p-Smad3 | Abcam, ab193297 | Rabbit-anti-human | WB |
| STAT3 | Abcam, ab68153 | Rabbit-anti-human | WB |
| p-STAT3 | Abcam, ab76315 | Rabbit-anti-human | WB |
| VEGF Receptor 1 | Abcam ab32152 | Rabbit-anti-human | WB |
| VEGF Receptor 2 | Abcam ab39638 | Rabbit-anti-human | WB |
| VEGF Receptor 3 | Abcam ab27278 | Rabbit-anti-human | WB |
| FGF Receptor 1 | Abcam ab76464 | Rabbit-anti-human | WB |
| FGF Receptor 2 | Abcam ab109372 | Rabbit-anti-human | WB |
| FGF Receptor 3 | Abcam ab133644 | Rabbit-anti-human | WB |
| PDGF Receptor alpha | Abcam ab203491 | Rabbit-anti-human | WB |
| PDGF Receptor beta | Abcam ab32570 | Rabbit-anti-human | WB |
| β-Actin | Abcam, ab8227 | Mouse-anti-human | WB |
| Secondary antibody | Abbkine, A21010; A21020 | Goat-anti-mouse/rabbit | WB |
| Type I Collagen | Servicebio, GB13022 | Rabbit-anti-human | IHC |
| FN | Abcam, ab45688 | Rabbit-anti-human | IHC |
| CD31 | Servicebio, GB13063 | Goat-anti-human | IHC |
| CD34 | Servicebio, GB13013 | Goat-anti-human | IHC |
| Secondary antibody | Servicebio, GB23204 | Rabbit-anti-goat | IHC |
| COL-1 | Abcam, ab6308 | Mouse-anti-human | IF |
| α-SMA | Abcam, ab5694 | Rabbit-anti-human | IF |
| Secondary antibody | Jackson ImmunoResearch, 111-095-003 | Goat-anti-rabbit | IF |