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. 2020 Apr 30;30(9):732–744. doi: 10.1038/s41422-020-0328-3

Fig. 5. ERCC6L2 and XLF are functionally redundant in DNA recombination.

Fig. 5

a Schematic illustration of V(D)J recombination screening procedure. Representative flow cytometry plot of chromosomal V(D)J recombination assay is showed. b Negatively enriched genes are showed for indicated genotypes. c Deficiencies of both ERCC6L2 and XLF cannot support efficient V(D)J recombination in a chromosomal V(D)J recombination assay. Representative flow cytometry plots of samples collected at Day 4 after STI-stimulation are showed at left and data from three replicates are summarized at right with mean ± SEM. Two-tail unpaired t-test was performed. **p < 0.01. d Southern blot analysis of INV substrate rearrangement products and intermediates. Genomic DNA was digested by Nde I/Nhe I (upper) or Nco I/Nhe I (lower), respectively. Unrearranged substrate (UR), coding end (CE) intermediates and coding join (CJ) are labeled.