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. 2020 May 26;14(9):2248–2260. doi: 10.1038/s41396-020-0682-7

Fig. 4. Analysis of the enzyme activity of TrpEG in the synthesis of anthranilic acid.

Fig. 4

a Genomic organization of the trpEG region in R. solanacearum GMI1000 (top). Domain structure analysis of TrpE (middle) and TrpG (bottom). b Detection of anthranilic acid production via the LC-MS assay. c SDS-PAGE analysis of the TrpEG protein. d Analysis of the production of anthranilic acid by TrpEG to catalyse chorismic acid transformation. The data are means ± standard deviations of three independent experiments. ***p < 0.001 (unpaired t test).