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. 2020 May 26;14(9):2248–2260. doi: 10.1038/s41396-020-0682-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Time-course analysis of anthranilic acid accumulation in the R. solanacearum wild-type strain (●) and the trpEG deletion mutant strain (■) and R. solanacearum cell growth in the wild-type strain (○) and the trpEG deletion mutant strain (□) in liquid medium. b β-Galactosidase activity of a trpEG-lacZ transcriptional fusion in the R. solanacearum wild-type strain (●) and the trpEG deletion mutant strain (▲) and the trpEG deletion mutant strain supplemented with anthranilic acid (■). The R. solanacearum GMI1000 strains were inoculated in a 2 L flask containing 1 L TTC medium and cultured at 28 °C with shaking at 220 rpm. The experiment was started at an initial OD₆₀₀ of 0.05. The data are means ± standard deviations of three independent experiments.