Fig. 3. Effect of rsiX deletion on eps expression in pellicles.
a Qualitative assessment of eps gene expression based on confocal laser scanning microscopy of pellicles formed by ∆rsiX showing high eps compared with WTanc. Cells constitutively expressing mKATE (OFF) are shown in magenta and eps-expressing cells (ON) are represented in green. Scale bar 10 µm. Right panel: average Peps-GFP expression levels in WTanc and ΔrsiX the WTanc calculated from mean values of Peps-GFP expression within ten single isolates. ***p < 0.001 (two-sample t-test). All data and corresponding p values are provided in Supplementary Data 1. b Flow cytometry results showing average distribution of fluorescence intensities of WTanc cells (dark gray), ∆rsiX cells (pink), and overlay of the two in comparison to WT non-labeled (light gray). (Color figure online).