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. 2020 Jun 1;14(9):2302–2312. doi: 10.1038/s41396-020-0685-4

Fig. 5. Effect of rsiX deletion on positioning of EPS producers in the pellicle.

Fig. 5

Competition assay between WT + Δeps and ΔrsiX + Δeps. Strains labeled with constitutively expressed GFP and mKate proteins were inoculated in 1:1 initial frequency, pellicles were cultivated for 48 h at 30 °C and visualized using stereomicroscope. Upper panels represent controls (two isogenic WT or ΔrsiX strains labeled with different fluorescent markers), middle panel represents pellicles formed by WT + Δeps, and bottom panels represent pellicles formed by ΔrsiX + Δeps, each in two alternative combinations of fluorescent markers. Scale bar corresponds to 500 µm.