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. 2020 May 26;30(10):914–927. doi: 10.1038/s41422-020-0341-6

Fig. 3. USP29 deficiency impairs HSV-1-triggered expression of downstream genes.

Fig. 3

a qRT-PCR analysis of Ifnb, Ip10, Il6, and Ccl5 mRNA in Usp29+/+ and Usp29m/m BMDMs infected with HSV-1 for 0–12 h, mock transfected (Mock) or transfected with DNA90, HSV60, or HSV120 for 6 h. b ELISA analysis of IFN-β, IFN-α, TNF, and CCL5 in the supernatants of Usp29+/+ and Usp29m/m BMDMs infected with HSV-1 for 0–24 h, or mock transfected (Mock) or transfected with DNA90, HSV60 or HSV120 for 12 h. c Immunoblot analysis of phosphorylated and total TBK1, IRF3 and IκBα, USP29 and GAPDH in Usp29+/+ and Usp29m/m BMDMs infected with HSV-1 for 0–6 h or transfected with ISD45 for 0–6 h. d Flow cytometry analysis (upper flow charts) and fluorescent microscopy imaging (lower images) of Usp29+/+ and Usp29m/m BMDMs infected with or without H129-G4 for 16 h. e qRT-PCR analysis of HSV-1 UL30 (upper) and plaque assays (bottom) of Usp29+/+ and Usp29m/m BMDMs infected with or without HSV-1 for 0–24 h. *P < 0.05; **P < 0.001; ***P < 0.001 (two-tailed Student’s t-test). Scale bars represent 300 μm. Data are representative of two (a, b, d, e) or three (c) independent experiments (means ± SD in a, b, e).