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. 2020 May 26;30(10):914–927. doi: 10.1038/s41422-020-0341-6

Fig. 7. Ablation of USP29 alleviates autoimmunity in Trex1−/− mice.

Fig. 7

a Immunoblot analysis(with anti-cGAS, anti-USP29, or anti-GAPDH) in heart (H), liver (L), kidney (K), or spleen (S) of Usp29+/+ and Usp29m/m mice (6-week old). b Representative image (upper image) and body weight (lower graph) of Trex1−/− and Trex1−/−Usp29m/m mice (5-week old). c Survival (Kaplan–Meier curve) of Trex1−/− (n = 38) and Trex1−/−Usp29m/m (n = 49) mice. d Immunoblot analysis (with anti-cGAS or anti-β-Actin) in brain (B), liver (L) or kidney (K) of Trex1−/− and Trex1−/−Usp29m/m mice (5-week old). e, f Representative image (e, left) and cell number (e, right) of spleens and HE staining of lungs and hearts (f) from Trex1−/− and Trex1−/−Usp29m/m mice (8-week old). g Flow cytometry analysis of spleenocytes from Trex1−/− and Trex1−/−Usp29m/m mice (8-week old) strained with fluorescence-conjugated antibodies against the indicated surface or intracellular molecules. h qRT-PCR analysis of Tnf, Il6, Il12p40 and Ifit3 mRNA in lungs and kidneys of Trex1−/− and Trex1−/−Usp29m/m mice (8-week old). i ELISA analysis of TNF, CCL5 and total IgG in the sera of Trex1−/− and Usp29m/m Trex1−/− mice (8-week old). *P < 0.05; **P < 0.001; ***P < 0.001 (log-rank analysis or two-tailed Student’s t-test). Data are representative of two independent experiments (means ± SD in b, e, g–i).