Fig. 4. Representative flow cytometry plots of lung single-cell suspensions from WT, SOD2^+/−, WT + CIH, and SOD2^+/− + CIH mice.

a The gating strategy for CD45⁺ myeloid lung cells to characterize pulmonary populations. The percentages of b monocytes (CD11b⁺ Ly6C^hi), c F4/80⁺ CD11c^– interstitial macrophages and d F4/80⁺ CD11c⁺ alveolar macrophages within the CD45⁺ Ly6G⁻ cell population in the lungs were statistically analyzed. The mean ± SEM is shown (n = 6 in each group). * P < 0.05 and **P < 0.01 compared with the WT group. CIH chronic intermittent hypoxia, IMs interstitial macrophages, AMs alveolar macrophages.