Fig. 7. Detection of mtROS and NLRP3 activation in LV-NC- or LV-shSOD2-transfected RAW264.7 cells incubated for 48 h under Nor or IH conditions with or without mito-TEMPO supplementation (100 nM).

a The percentage of mitoSOX-positive cells in the five groups was detected by FACS analysis. b RT-PCR results for IL-1β in cell lysates are shown. c Supernatants were analyzed for IL-1β by ELISA. d The protein expression of NLRP3 and ASC was detected by Western blot analysis. e Quantification of relative protein expression was performed by densitometric analysis, and GAPDH acted as an internal control. All data are presented as the mean ± SEM. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with the control group. Every experiment was repeated at least three times. RT-PCR quantitative reverse transcriptase-polymerase chain reaction, IH intermittent hypoxia.