Figure 1.
High level of circRAD18 in AML patients and cells. (A) The level of circRAD18 in the blood samples from 55 AML patients and 55 healthy donors was examined with qRT-PCR analysis. (B) The level of circRAD18 in HS-5, THP-1 and HL-60 cells was determined by qRT-PCR assay. (C and D) After total RNA in THP-1 and HL-60 cells was treated with or without RNase R, circRAD18 and linear RAD18 levels were detected via qRT-PCR assay. (E) The expression level of circRAD18 in THP-1 and HL-60 cells transfected with si-NC, si-circRAD18#1 or si-circRAD18#2 was determined with qRT-PCR assay. *P<0.05.