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. 2020 Oct 30;12:10887–10896. doi: 10.2147/CMAR.S277432

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© 2020 Wang et al.

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Effects of circRAD18 knockdown on AML cell biological behaviors. (A and B) The proliferation, (C) colony formation and (D) apoptosis of THP-1 and HL-60 cells were assessed by CCK-8 assay, colony formation assay and flow cytometry analysis, respectively. (E and F) The protein levels of Bax, Bcl-2 and c-caspase-3 in THP-1 and HL-60 cells were measured by Western blot assay. (G and H) The cell cycle process in THP-1 and HL-60 cells was analyzed by flow cytometry analysis. (I and J) The migration and invasion of THP-1 and HL-60 cells were explored by transwell assay. *P<0.05.