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. 2020 Oct 30;12:10887–10896. doi: 10.2147/CMAR.S277432

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© 2020 Wang et al.

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MiR-206 directly targeted PRKACB in AML cells. (A) The predicted complementary sequences between miR-206 and PRKACB. (B and C) The interaction between miR-206 and PRKACB in THP-1 and HL-60 cells was analyzed by dual-luciferase reporter assay. (D) The database TCGA showed PRKACB expression was upregulated in AML patients. (E–H) The mRNA and protein levels of PRKACB in AML patients’ blood samples and AML cells were measured by qRT-PCR assay and Western blot assay, respectively. (I–K) After THP-1 and HL-60 cells were transfected with miR-NC or miR-206, the levels of miR-206, PRKACB mRNA and PRKACB protein were determined by qRT-PCR assay or Western blot assay. (L and M) The mRNA and protein levels of PRKACB in THP-1 and HL-60 cells transfected with si-NC, si-circRAD18, si-circRAD18+anti-miR-NC or si-circRAD18+anti-miR-206 were detected using qRT-PCR assay and Western blot assay, respectively. *P<0.05.