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. 2020 Nov 2;10:18859. doi: 10.1038/s41598-020-75244-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of Thymus albicans essential oil on the iNOS and COX-2 protein levels in LPS stimulated RAW 264.7 macrophages. Cells (1.2 × 10⁶ cells/well) were kept 24 h in medium (control) or pre-treated for 1 h with 0.64 μL/mL of T. albicans essential oil and then stimulated with LPS (1 μg/mL). Total cell lysates were analyzed by western blot. Results are expressed as protein levels compared to LPS. Statistical differences were calculated by two-tailed unpaired student’s t-test (###p < 0.001, compared to control; **p < 0.01, ***p < 0.001, compared to LPS).