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. 2020 Nov 2;10:18872. doi: 10.1038/s41598-020-75885-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of chronic heat stress on the protein expression in the ileum of chicken (n = 8). (A) Mean relative expression of HSP70, (B) mean relative expression of HSP60, (C) mean relative expression of HSP47, (D) western blot band analysis (cropped from same gel). ^A–CDifferent superscripts show significant differences (P < 0.05) among different groups on day 35. Asterisk (*) represents statistical difference between different treatment groups on day 28 and 35, *P < 0.05, **P < 0.01. We did not use the original blot in the manuscript, because it is very difficult to find out appropriate blot from the fuller length blot. The complete blots of the ileum are presented in supplementary materials “Figure S9”.