Table 2.

Primer sequences of genes assessed in the colon tissues of female CD-1 mice using real-time quantitative PCR analysis.

Target gene	Sense primer (5′ → 3′)	Antisense primer (5′ → 3′)	Full name
Inflammation
Ifng	TCATGGCTGTTTCTGGCTGT	CACCATCCTTTTGCCAGTTCC	Interferon gamma
Tnf	AGCCCACGTCGTAGCAAAC	TTTGAGATCCATGCCGTTGG	Tumor necrosis factor
Il4	CTCGAATGTACCAGGAGCCA	GTGGTGTTCTTCGTTGCTGT	Interleukin 4
Il5	TGACTCTCAGCTGTGTCTGG	AAGCCTCATCGTCTCATTGCT	Interleukin 5
Il6	ACAAAGCCAGAGTCCTTCAGAG	AGAGCATTGGAAATTGGGGT	Interleukin 6
Il13	CTCTTGCTTGCCTTGGTGGT	CTCCATACCATGCTGCCGTT	Interleukin 13
Il17a	GCCCTCAGACTACCTCAACC	CAGCTTTCCCTCCGCATTGA	Interleukin 17A
Gut integrity
Zo-1	ATGTTTATGCGGACGGTGG	TTTCCTCCATTGCTGTGCTCTTA	Zonula occludens-1
Zo-2	GCAGCTTGTAGTTCTGAGCCG	TACTGCTCCCATATCACCTCCT	Zonula occludens-2
Zo-3	AAGTGGGGGCTGATTGTTTCCA	AGTGTGGCTGTGTGTTGTTCC	Zonula occludens-3
Ocln	GGTCCTCCTGGCTCAGTTG	AAGATAAGCGAACCTTGGCGG	Occludin
Cldn1	TCTACGAGGGACTGTGGATGT	ATTACCATCAAGGCTCGGGT	Claudin 1
Cldn4	AGCAAACGTCCACTGTCCTT	GGGGCGTAATGGCAAGAGTA	Claudin 4
Cell regulators/proliferation/apoptosis
Bcl2	ATGCCTTTGTGGAACTATATGGC	GGTATGCACCCAGAGTGATGC	B-cell lymphoma 2
Aifm1	AGGACGGTGAGCAACATGAA	GTTCTATCCACCCATCCCGC	Apoptosis-inducing factor 1
Bcl2l10	CGCTACACACACTGACTGGA	CTTTAGGATCCCCTGCCCTG	B-cell lymphoma 2 like 10
Ccnb1	TGCATTCTCTCAGTGCCCTCCACA	AGACAGGAGTGGCGCCTTGGT	Cyclin B1
Ccnd2	CCTTTGACGCAGGCTCCCTTCT	ACCCTGGTGCACGCATGCAAA	Cyclin D2
Ccna2	GCTCTACTGCCCGGAGGCTGA	TGGCCTACATGTCCTCTGGGGAA	Cyclin A2
Ccne1	GGTGTCCTCGCTGCTTCTGCTT	CCGGATAACCATGGCGAACGGA	Cyclin E1
Cdkn1a	TTAGGCAGCTCCAGTGGCAACC	ACCCCCACCACCACACACCATA	Cyclin dependent kinase inhibitor 1A
Cdk4	AGAAACCCTCGCTGAAGCGGCA	TGGGGGTGAACCTCGTAAGGAGA	Cyclin dependent kinase 4
Ki67	GCTCACCTGGTCACCATCAA	ACTACAGGCAGCTGGATACG	Kiel 67
Housekeeping genes
$\beta$-Actin	GGGCACAGTGTGGGTGAC	CTGGCACCACACCTTCTAC	Actin, beta