Figure 4.

The MLL4-HMG-USD domain rescues some gene expression via recruiting UTX to chromatin. (A) In ΔMLL4-T/O cells, ChIP-seq was performed for GFP-HMG-USD and UTX using antibodies recognizing GFP and UTX, respectively, after 0, 2, and 4 d of doxycycline induction. HMG-USD and UTX ChIP-seq average peaks (top) and heat maps (bottom) are centered at HMG-USD dox day 4 peaks and presented as log₂FC. (B) After 8 d of dox induction, 475 genes are differentially expressed compared with uninduced ΔMLL4. Relative expression levels are hierarchically clustered and presented as Z-score heat maps for the same 475 genes across all five conditions. Note that the day8 induction time point bears closer resemblance to WT, indicating that a fraction of MLL4-dependent genes are “rescued” by sustained HMG-USD expression.