Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 1;34(21-22):1503–1519. doi: 10.1101/gad.340216.120

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Ramamoorthy et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 3. — The activation of Myc and its targets in dHet B-ALL. (A) Overlap of genes differentially expressed (X-axis) and differentially bound by EBF1 (Y-axis) in dHet B-ALL cells relative to wild-type pro-B cells (left), dHet B-ALL cells relative to dHet pro-B cells (middle), and dHet pro-B cells relative to wild-type pro-B cells (right). The key differentially regulated genes between dHet B-ALL and wt pro-B cells are highlighted. (B) The abundance of the Myc-binding motif in the B-ALL gained ATAC peaks (top) and digital genomic footprinting analysis showing normalized Tn5 insertion profiles, in dHet B-ALL and wt pro-B cells, around footprinted Myc motifs identified in dHet B-ALL cells (bottom). Insertions on the forward and reverse strands are indicated in red and blue, respectively. The percentage of peaks having the motif, the P-value, and percentage detected in the background are indicated. (C,D) Screenshots showing the distribution of EBF1 and Pax5 occupancy and chromatin accessibility, as determined by ChIP-seq and ATAC-seq analysis, on the Bcl6 gene (C, left), Foxo1 gene (C, right), and BENC regulatory elements of the Myc locus (D) in wt pro-B, dHet pro-B, and dHet B-ALL cells. The ChIP and ATAC signals are normalized to 10 million reads (Y-axis). (E) Heat map of Myc ChIP signals, from the wt, preleukemic (Eµ-Myc^tg), and leukemic cells (Eµ-Myc^tg), 2 kb around the center of dHet B-ALL-lost and B-ALL-gained ATAC peaks. (F) Overlap of genes up-regulated (top) or down-regulated (bottom) in dHet B-ALL cells to the genes differentially expressed in the Eµ-Myc^tg leukemic cells. The differentially expressed genes are ranked according to the fold change difference between Eµ-Myc^tg leukemic cells and wt cells.