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. 2020 Oct 28;6(44):eabd2688. doi: 10.1126/sciadv.abd2688

Fig. 4. DRB1*04:01 (DR4) transgenic mice that receive intra-articular injections of cNETs develop ACPAs and anti-CarP.

Fig. 4

(A) Rh-PG probe against citrulline was used to detect specific citrullinated proteins in NETs (PMA) and cNETs (PMA + CN). (B) Western blot analysis against carbamylated proteins was performed in NET and cNETs. (C) Densitometry analysis of citrullinated histone H3 in NETs generated with PMA or PMA + CN (n = 4 per group). Results are the mean ± SEM. Mann-Whitney U test was used. *P < 0.05. (D) Serum ACPAs and (E and F) anti-CarP antibody levels were measured at various points in animals immunized with NETs, cNETs, or spontaneously generated RA-NETs (n = 4 to 8 per group). Results are the mean ± SEM. Kruskal-Wallis test was used. **P < 0.01 and ***P < 0.001.