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. 2020 Oct 30;6(44):eabc1799. doi: 10.1126/sciadv.abc1799

Fig. 5. Hh signaling is necessary for tendon enthesis responses to mechanical loading.

Fig. 5

(A) Experimental design. (B) Immunofluorescence of primary cilia (red, white arrowheads) and the Hh component Smo, (green) from 9-week-old ScxCre;Smofl/fl mice (cKO) and their WT littermates. (C) Toluidine blue staining of the tendon entheses from 13-week-old WT and cKO mice after cage activity (control), BtxA-induced muscle paralysis (unloading), and treadmill running (overloading). Dashed lines mark the tendon enthesis. (D to H) Primary cilium incidence at the tendon enthesis (D) of WT and cKO mice subjected to cage activity (control), unloading, and overloading conditions; tendon cross-sectional area (E); tendon enthesis mechanical properties (F); bone quality of the humeral head (G); analysis of walking patterns (H). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. IHC, immunohistochemistry.