Fig. 2. In vitro characterizations of Iod-CBT-NPs.

(A) TEM image of Iod-CBT-NPs. (B) HPLC traces of 500 μM NBC-Iod-CBT (black), 500 μM NBC-Iod-CBT incubated with TCEP (2 mM) for 1 hour, and a further incubation with NADH (5 mM) and NTR (5 U/ml) for 2 hours in 10 mM PBS at 37°C (red). Wavelength for detection: 320 nm. (C) 2D projection image of Iod-CBT-NPs. (D) 3D rendering image of Iod-CBT-NPs (yellow). LAC, linear absorption coefficient.