Table 2. Binding affinities of CaCohA2 and CaDoc0917 with mutations measured by ITC.

CaCohA2	CaDoc0917	KA (M−1) at pH 4.8	KA (M−1) at pH 7.5	Affinity ratio
Wild type	R49D	4.3 ± 0.3 × 107	13.9 ± 0.4 × 105	31
E128Q	R49D	2.1 ± 0.2 × 107	15.7 ± 0.7 × 105	13
D130N	R49D	2.1 ± 0.3 × 107	42.4 ± 1.8 × 105	5
E128QD130N	R49D	1.1 ± 0.1 × 107	23.2 ± 1.3 × 105	5
Wild type	R49DV19M	51.5 ± 21.9 × 107	19.8 ± 2.4 × 105	260
Wild type	R49DR22I	2.1 ± 0.2 × 107	1.8 ± 0.3 × 105	117
Wild type	R16D	10.3 ± 1.6 × 107	1.7 ± 0.3 × 105	606
E128Q	R16D	2.2 ± 0.3 × 107	1.2 ± 1.0 × 105	183
D130N	R16D	0.8 ± 0.1 × 107	3.2 ± 0.7 × 105	25
E128QD130N	R16D	0.36 ± 0.03 × 107	4.6 ± 0.3 × 105	8
Wild type	R16DM52V	2.9 ± 0.4 × 107	2.9 ± 0.5 × 105	100
Wild type	R16DI55R	13.2 ± 1.5 × 107	10.4 ± 0.5 × 105	127
Wild type	R16DE53Q	5.0 ± 0.9 × 107	19.3 ± 1.3 × 105	26