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. 2020 Oct 23;6(43):eaba0942. doi: 10.1126/sciadv.aba0942

Fig. 2. Antibacterial efficacy of L-AgÅPs-gel in vitro.

Fig. 2

(A) Zones of inhibition surrounding the blank-gel–, L-AgÅPs-gel–, or AgNPs-gel–infused paper disks against S. aureus ATCC25923, S. aureus ATCC29213, and P. aeruginosa ATCC27853 on agar plates. (B) Quantification of the average diameters of inhibition zones. n = 3 per group. 1 – β = 1. (C) Digital photos of bacterial colonies grown on agar plates in different treatment groups. (D) Quantification of the numbers of bacterial colonies in (C). n = 3 per group. 1 − β = 1. CFU, colony-forming units. (E and F) Calcein-AM/PI staining images of the above bacteria receiving different treatments for 3 hours (E) and quantification of the ratios of live bacteria [calcein-AM+PI−; (F)]. Scale bar, 10 μm. n = 3 per group. 1 − β = 1. (G) Cell viability analysis of bacteria in presence of blank-gel, L-AgÅPs-gel, or AgNPs-gel for 3 hours by alamar blue assay. n = 3 per group. 1 − β = 1. (H) Absorbance of the crystal violet–stained biofilms formed by bacteria treated with blank-gel, L-AgÅPs-gel, or AgNPs-gel for 36 hours. n = 3 per group. 1 − β = 1. (I) Absorbance of the crystal violet–stained survived biofilms exposed to blank-gel, L-AgÅPs-gel, or AgNPs-gel for 36 hours. n = 3 per group. 1 − β = 1. (J) TEM images of ultrathin sections of different bacteria receiving different treatments for 3 hours. Scale bar, 500 nm. *P < 0.05, **P < 0.01, and ***P < 0.001. Photo credit: Chun-Yuan Chen and Hao Yin, Central South University.