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. 2020 Oct 23;6(43):eabb7107. doi: 10.1126/sciadv.abb7107

Fig. 1. CRISPR-Cas9–mediated knockin of HBB/GFP gene.

Fig. 1

(A) Schematic of the mechanism governing the combined SMNP/SNSMD strategy for CRISPR-Cas9–mediated knockin of HBB/green fluorescent protein (GFP) gene into K562 3.21 (SCD) cells via two consecutive steps. (B) A self-assembled synthetic approach for the preparation of Cas9•sgRNA⊂SMNPs through stoichiometric mixing of Cas9•sgRNA and the four molecular building blocks. (C) A self-assembled synthetic approach for the preparation of HBB/GFP plasmid⊂SMNPs.