Fig. 2. The combined SMNP/SNSMD strategy for delivering EGFP-Cas9•sgRNA or HBB/GFP plasmid into K562 3.21 cells.
(A) The combined SMNP/SNSMD strategy for delivering EGFP-Cas9•sgRNA into K562 3.21 cells. (B) Three batches (15 formulations) of EGFP-Cas9•sgRNA⊂SMNPs were prepared for delivering EGFP-Cas9•sgRNA into K562 3.21 cells to identify an optimal formulation (marked with an asterisk). (C) The combined SMNP/SNSMD strategy for delivering HBB/GFP plasmid into K562 3.21 cells. (D) Three batches (15 formulations) of HBB/GFP plasmid⊂SMNPs were prepared for GFP transfection studies to identify an optimal formulation (marked with an asterisk).