| Data collection 3D reconstruction |
| Facility |
Boulder EM Services |
Janelia |
| Microscope / detector |
Tecnai F30 / K2 |
Titan Krios / K2 |
| Acceleration voltage (kV) |
300 |
300 |
| Magnification |
31 000x |
22 500x |
| Super-resolution mode |
no |
yes |
| Physical pixel size (Å/px) |
1.271 |
1.31 |
| Nominal defocus range (μm) |
-1 to -2.5 |
-1 to -2.5 |
| Electron dose rate (e-/px/s) |
17.83 |
10 |
| Exposure time (s) |
4 |
8 |
| Total electron dose (e-/Å2) |
56.10 |
58 |
| Number of movie frames |
40 |
50 |
| Dose per frame (e-/Å2) |
1.4025 |
1.16 |
| Number of movies collected |
632 |
2 355 |
| Initial number of particles |
226 250 |
984 171 |
| Number of particles for 3D classification |
168 765 |
983 946 |
| Symmetry |
C1 |
C1 |
| Number of particles for 3D refinement |
27 889 |
16 304 |
| Resolution at FSC = 0.143 |
10.5 / 11.6 |
10.5 / 12.3 |
| (masked / unmasked) (Å) |
| B factor for map sharpening (Å2) |
-1 127.02 |
-833.594 |
| Model refinement |
| Chains |
22 |
|
| Atoms |
26 958 |
|
| Amino acid residues |
1735 |
|
| Nucleotides |
638 |
|
| Water molecules |
0 |
|
| Bond length RMSD (Å) (number of outliers > 4σ) |
0.015 |
|
| (1) |
| Bond angle RMSD (°) (number of outliers > 4 σ) |
1.938 |
|
| (187) |
| MolProbity score |
0.56 |
|
| Clash score |
0.14 |
|
| Ramachandran outliers (%) |
0.00 |
|
| Ramachandran allowed (%) |
1.59 |
|
| Ramachandran favored (%) |
98.41 |
|
| Rotamer outliers (%) |
0.21 |
|
| Cβ outliers (%) |
0.00 |
|
| Peptide plane outliers (%) |
0.00 / 0.00 |
|
| Cis proline / general |
| Peptide plane outliers (%) |
0.00 / 0.00 |
|
| Twisted proline / general |
| CaBLAM outliers (%) |
0.78 |
|
| Protein B factors (Å2) |
24.17 / 521.23 / 180.96 |
|
| min/max /mean |
| DNA B factors (Å2) |
60.99 / 771.83 / 271.27 |
|
| (min/max/mean) |
| Model-to-map real-space CC |
0.61 |
|
| (mask) |
| Model-to-map real-space CC |
0.79 |
|
| (box) |
| Model-to-map real-space CC |
0.42 |
|
| (peaks) |
| Model-to-map real-space CC |
0.53 |
|
| (volume) |
