Figure 4.

Concomitant emergence of activated cT_FH and B cells in blood is reflective of increased GC activity.ctivated B cells emerge concomitantly in blood and reflect active GC responses. Flow cytometry analysis of blood B cells in patients analyzed for cT_FH in Figure 1F. (A) Representative examples of flow cytometry analysis and dot plots of percentages of Ki67⁺IgD⁻ among CD27⁺ cells are displayed for DSA⁻ (n=48), DSA⁺ABMR⁻ (n=27) and DSA⁺ABMR⁺ (n=20) groups. (B) Representative flow cytometry analysis and percentages of Ki67⁺IgD⁻CD20⁺CD38^lo ABCs and Ki67⁺IgD⁻CD20⁻CD38^hi ASCs among CD27⁺ cells are displayed for DSA⁻ (n=48), DSA⁺ABMR⁻ (n=27) and DSA⁺ABMR⁺ (n=20) groups. (C) Plasma CXCL13 levels measured by ELISA for DSA⁻ (n=28), DSA⁺ABMR⁻ (n=25), and DSA⁺ABMR⁺ (n=20) groups. (D) Spearman correlation analysis of Ki67⁺ICOS⁺ cT_FH and ABCs with plasma CXCL13 levels for DSA⁻ (n=28), DSA⁺ABMR⁻ (n=25), and DSA⁺ABMR⁺ (n=20) groups. Mann-Whitney U test was used for (A–C). *P<0.05; **P<0.01; ****P<0.001. Each dot represents one patient.