Figure 7.

Dynamics of cT_FH and ABC responses correlate with timing of ABMR onset. Patients were sampled longitudinally from pretransplant to the indicated intervals. (A) t-SNE projections of cT_FH cells from three individual patients, overlaid with those that are Ki67⁺ICOS⁺. Each t-SNE map is on the basis of n=3000 cells per time point. Pink arrows indicate the time point of onset of ABMR. (B) Kinetics of emergence of Ki67⁺ICOS⁺ cT_FH (connecting lines) and DSAs (gray bars) in indicated patient groups: DSA⁻ (n=5), DSA⁺ABMR⁻ early (n=5), DSA⁺ABMR⁻ late (n=2), DSA⁺ABMR⁺ early (n=2), and DSA⁺ABMR⁺ late (n=2). Mixed-effects model for comparison of %Ki67⁺ICOS⁺ cT_FH between DSA⁺ABMR⁺ or DSA⁺ABMR⁻ groups with the DSA⁻ group. *P<0.05; **P<0.01; ****P<0.001. (C) Kinetics of cT_FH, ABCs, and ASCs in patient groups, as in (B). Blue arrows indicate the time of DSA emergence post-transplant and pink arrows indicate the time point of ABMR onset. cT_FH, ABC, and ASC data are shown as percentage±SEM and DSA bars represent mean values.