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. 2020 Jul 31;31(10):2372–2391. doi: 10.1681/ASN.2019121261

Figure 4.

Figure 4.

LIM-nebulette expression is significantly reduced in human disease at both transcript and protein level. (A) Mining of the NephroSeq database shows that mRNA expression levels of NEBL are significantly reduced in both DN and FSGS cases (**P<0.01, ****P<0.001; nonparametric unpaired t test, n=8–13 cases in each group). (B) Representative images that show the spatial protein expression of LIM-nebulette (and actinin-4 as positive control) from healthy nephrectomy versus DN and FSGS cases after formalin fixation, paraffin embedding, and immunofluorescent staining using antigen retrieval (scale bars, 50 μm). (C) Protein expression levels as estimated by the percentage of glomerular area positively stained after segmentation and grayscale thresholding (*P<0.05, ***P<0.001, ****P<0.001; one-way ANOVA with post hoc Tukey test, n=4 cases with 20–34 glomeruli in each group).