Figure 3.

The Hippo pathway is required for podocyte response to injury. (A) Immunoblots showing increased protein levels of TAZ and YAP in glomerular lysates 2 days after nephrotoxic injury. Blots are representative of two independent experiments. Vinculin was used as loading control. (B) YAP/TAZ target genes are induced in podocytes after nephrotoxic injury. Normalized expression levels are shown as violin plots. (C) Deletion of TAZ (Wwtr1^−/−) or YAP (Yap1^−/−) exacerbates proteinuria. Albumin-creatinine ratios (ACR) were measured from spot urine collected from mice at the indicated time points after nephrotoxic serum injection. (D) Kidney sections were stained with PAS and Masson trichrome stain 5 weeks after injection with nephrotoxic serum or saline and scored blindly for GN and fibrosis. Scoring criteria are described in the methods. Results were analyzed by multiple t tests using the two-stage linear step-up method (*q<0.05, **q<0.01, ****q<0.0001). WT, wild type.