Figure 5.

PKD1 mutant UB organoids exhibit cystogenesis upon forskolin treatment. (A) Outline of the induction protocol for UB organoids with forskolin treatment. At day 18.5, forskolin was added at a final concentration of 25 μM. (B) Time-course bright-field images of UB organoids upon forskolin treatment. Scale bars, 200 μm. (C) Frequency of cyst formation in UB organoids (two clones per genotype; n=4 per clone in five independent experiments). (D) Cyst areas in UB organoids (n=20 per clone). Data are shown as mean±SEM. *P<0.05, **P<0.01.